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One of the trends that we see coming from this rediscovery of virtualization technology is the virtualization of service quality. In the past, the levels of service afforded to different critical applications would generally be defined haphazardly for each application. The use of backups, mirroring, clustering, replication, and other data and system protection methods would be applied to different applications as they were needed, but with little or no consistency from one application to the next. Under virtualized Quality of Storage (QoS), an enterprise clearly defines a few (often three or four) levels of service that it wishes to make available to its applications. For our purposes, consider the service levels shown in Table 8.1. We have defined three levels of service, which we call Gold, Silver, and Bronze, and we have assigned each of our system applications to one of these levels of service.

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This method has been developed into a two-site sandwich assay for digoxin, using phycoerythrin as F1 and uorescein as F2.15 Phycoerythrin is isolated from red algae, has a quantum yield of 0.80, and absorbs 30 times more light than does uorescein. Phycoerythrin-labeled primary antibody is immobilized onto microtiter plate wells. Following incubation with the digoxin sample to allow quantitative binding, uorescein-labeled second antibody is added. Fluorescein quenches the emission from phycoerythrin, as shown in Figure 6.6, so that greater quantities of immobilized digoxin yield reduced emission intensities. This assay has a detection limit of 0.5-mg/L digoxin, with a 6% coef cient of variation: 6.3.4.5. Time-Resolved Fluorescence. This instrumental technique may be applied to most immunoassays that require uorescence intensity measurements,

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Table 8.1 An Example of Virtualized Levels of Service SYSTEM PROTECTION BACKUPS Daily fulls, with replicated indexes Weekly fulls, nightly incrementals Monthly fulls and weekly incrementals APPLICATIONS ERP, CRM, customerfacing e-commerce E-mail, software development, accounts receivable Accounts payable, payroll, marketing Clustered locally and remotely Clustered locally RELATIVE COST $$$$$ $$$

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but do not require antibodies to the uorophore itself. Its popularity stems from the rejection of interfering uorescent signals and scattered excitation radiation. If a mixture of short- and long-lived uorophores is excited with a short (<1 ms) pulse of light, then the excited molecules will emit uorescence that is either short- or long lived. In both cases, uorescence will decay exponentially with time, but short-lived uorescence will decay to nearly zero very quickly. If emission measurements are taken after 100 200 ms, only long-lived uorescence will be measured. Short-lived uorescence and scattered light may thus be eliminated from measured signals. The labeling uorophore must possess a long-lived excited state, and only a few species, notably europium chelates, have suitable uorescence properties.16 Proteins labeled with p-isothiocyanatophenyl EDTA at lysine residues will chelate Eu3 with formation constants in excess of 1012 M 1 . Typically, a 1 ms excitation pulse at 337 nm is followed by a 200-ms delay, following which emission at 613 nm is integrated between 200 and 600 ms. Background long-lived uorescence from polystyrene (used for microtiter plates) is extremely low, but other solid-phase supports, such as nitrocellulose and 3M paper, exhibit signi cant long-lived background signals. 6.3.5. Chemiluminescent Labels Chemiluminescent labels may be employed in sandwich or competitive antigen assays. In sandwich assays, a solid support holds a primary antibody, and incubation with ligand yields a species that is detectable following a second incubation step with a labeled second antibody. Luminol has been tested as an immunoassay label; it may be coupled to proteins through its primary amino group. Luminol reacts with hydrogen peroxide and hydroxide in a microperoxidase-catalyzed reaction, which yields light at 430 nm (Eq. 6.8):

RAID-5

1123 Implementing the pilot: first difficulties When the choice of vendor was confirmed, an initial, pilot project was selected At a functional level, very few concerns emerged; parameters for the transformation functions were easily set using the tool A first obstacle: the chosen applications were in fact exchanging information in files In the time available, it was neither functionally necessary nor technically possible to modify them to produce and/or receive messages In addition, the EAI vendor provided a file adapter (see section 3132) that could read or write the file upstream/downstream from its integration engine Then, a second concern: for the slightly older existing applications, most file record formats used fixed position/length, though some used a variable format of indicator mnemonic value .

8

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We have, of course, chosen these levels of service and assigned these applications to them rather arbitrarily. Different organizations will have wildly differing requirements. The point is that by virtualizing these levels of service, a technique that is made easier with SAN-virtualized storage, it becomes much easier to set and deliver service levels across the enterprise. It s also easier to determine how much each application must pay the system administrative staff to deliver their required levels of service. Obviously, gold service will cost a great deal more than silver or bronze, but for applications like ERP and CRM, which can be the lifeblood of the organizations who run them, it is worth the added expense to keep those applications running a greater percentage of the time.

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