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A recently introduced chemiluminescent labeling system employs arylacridinium esters17 as labels that are readily coupled to protein tyrosine residues. In the presence of hydroxide and H2O2, the ester is oxidatively cleaved, yielding 10-methylacridone plus light (Eq. 6.9). This reaction does not require an enzymatic catalyst, and quantum yields are high even when the label is attached to proteins, since quenching by hapten or protein is minimized by the release of the excited acridone. Nearly all light is given off within 10 s after oxidation, and so high S/N values are achievable. Furthermore, antibodies can be labeled to such an extent that speci c activities of 1015 photons are obtained per gram of antibody. However, the phenyl ester bond is inherently unstable to hydrolysis, so that labeled reagents have a limited shelf-life. Research toward more stable acridinium esters is underway. asp.net pdf 417 ASP . NET PDF-417 Barcode Generator - Generate 2D PDF417 in ...
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Easy-to-use ASP . NET PDF417 Barcode Component, generating PDF-417 barcode images in ASP.NET, C#, VB.NET, and IIS project. We looked at issues for desktop applications that use databases in the previous chapter. On the server side, a database application needs to be able to recover itself, often independently of the database server. Server-based applications may need to notify the users of failures that require manual intervention, so recovery requires coordination of the server-resident and desktop-resident application components. If you re using a browser-based (HTML client) front end to your application, then generation of the appropriate error and user information pages is the responsibility of the server-side application alone. 6:9 asp.net pdf 417 PDF417 ASP . NET - Barcode Tools
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