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PROBLEMS 1. (a) Calculate the RCF maximum (RCFmax), minimum (RCFmin), and average (RCFav), for a centrifugation tube in which the meniscus is 12 cm from the rotation axis and the bottom at 22 cm. The rotor is driven at 27,000 rpm. (b) If the rotor is driven at half-velocity (13,500 rpm) will the g force applied to the solution be approximately one-half 2. (a) A titanium xed-angle rotor can be run at a maximum speed of 52,000 rpm. It has rmax 10.8 cm and rmin 3.2 cm. Calculate the k (clearing) value. (b) Compare the obtained value with the ones presented in Table 13.2. Choose the best rotor for the separation of soluble proteins from a mammalian cell homogenate. 3. A vertical rotor data sheet speci es k 304. The tubes for this rotor have an internal diameter of 2.4 cm, with rmin 13.6 cm. What is the maximum speed for this rotor (Note that in real situations, aging of the rotor and accumulation

What are the delivery semantics Do you get delivery of messages at least once, at most once, or exactly once At least once means you may get duplicate messages if the middleware believes a message was lost on the network. At most once means you have no duplicates, but lose messages that are eaten by the network or by overloaded servers. Exactly once is the transactional, ACID-property-friendly behavior that s ideal, but often the slowest and hardest to guarantee. (ACID properties are covered in detail in the Database Servers section in 14, Data and Web Services. ) Deal with duplicate messages. If you may get duplicates, make sure you know how to suppress them or filter them out. In some cases, duplicates are immaterial and can be ignored. If you get the same quote on a stock once or five times, only the last reported quote really matters. Similarly, if you run the risk of falling behind in processing state updates, determine if you can compress the updates and take the last one or the net-net change of all of them. Being flexible with the update stream allows processes to pick up and remove slack from the distribution system. What s the latency under load As with queuing systems, you want to be sure the distribution middleware holds up with increasing request volumes.

TABLE 13.2. Values of k and Total Capacity for Ultraspeed Rotorsa Rotor Type Swinging-bucket Swinging-bucket Swinging-bucket Fixed-angle (18 ) Fixed-angle (14 ) Fixed-angle (29 ) Vertical Vertical Vertical

As you compare various state replication techniques and application issues to handle duplicates, lost messages, and updates, the process replication problem bears an uncanny resemblance to a transaction replication problem. The same concepts are involved, and the same constraints and concerns should guide your selection of technologies and designs. With some good groundwork and developer guidance, you can build a system that tolerates failures at any level, from the hardware to the application coding.

Not all processes handle short-lived requests Reliability engineering also has a place in the scientific and modeling worlds, where codes may run for several hours, days, or weeks Taking a hit because of insufficient memory or a network error in the middle of a weeklong simulation run incurs about 50 hours of recovery time to get the simulation back to the point of failure Given that most simulations are run to reduce time to market, wasted simulation cycles and dead time are not easily forgiven You need to add reliability at the process level to ensure rapid recovery of long-running jobs.

RCFmax (g) 90,300 285,000 484,200 59,200 94,500 511,000 70,000 240,600 510,000

Checkpointing can be done at the application level, with extra code to handle writing out critical state information; it can be done using a job scheduler or tool such as the Load Share Facility (LSF, from Platform Computing), or it can rely on operating system support for preserving an entire process and its in-memory state at any point in time Checkpoint-restart facilities are not common, so we ll stop at this brief mention of them All of the techniques and issues described in the following text apply to OS-level support as well, but we re going to focus on more application-controlled techniques to allow restart and recovery What kind of failures scare the scientific coder the most Obviously, resource shortages such as memory, swap space, and filesystem space represent threats to the completion of a long-running code.