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Give them interesting work Don t micromanage Ask them what makes them happy, and try to deliver it Let them decorate their cubes Keep some simple food and drink around for late-night work Don t make them dress up Relaxed As long as the systems are running well, there s nothing wrong with letting them occasionally surf the Web or maintain a personal web page during the day if they want to (Remember all those late nights and weekends!) In 5 we talked about the six computing environments you might need, with the last one being the playground Don t be afraid to let your system staff run free in the playground, because they may just come up with the next big process improvement..

Both primary and secondary Ag Ab interactions may be exploited for analytical purposes, but quantitative bioanalytical methods make extensive use of primary binding interactions. The intrinsic association constant that characterizes antibody epitope(hapten) binding is called the af nity: ! Ab H Ab : H Affinity K Ab : H = Ab H 5:3 5:4

Responsible. If your SA is responsible for the proper functioning of a particular system, then she needs the authority to change things as she sees fit. It s not fair or reasonable to assign responsibility without granting the authority that goes with it. Interested. Make the work challenging and fun. Make the workplace a pleasant place to spend time. Keep things as high-tech as possible. Motivated. Reward good work. Let your SAs have cutting-edge equipment and software if they are good at what they do. Feed them; there s no better way to get late-night work out of them. Well compensated. If you won t, somebody else will! Top-grade system administrators are very hard to find and well worth the extra money that they cost. Educated. Send them to training and conferences like USENIX and LISA, and user conferences, regularly. Let them stay on the cutting edge of technology. There are entire user groups, like the 24 7 Users Group, dedicated to system and physical plant availability. Well read. Get them the magazines and books that they need to keep up on current trends in technology. One good tip from a magazine will pay for the magazine for many years to come. Around! If you have frequent personnel turnover, it will be very difficult to maintain your system availability. Don t undervalue your important people. Make sure they want to keep working for you.

System administration is a 24 7 job. Unless someone is on-site 24 hours a day, as is realistic in larger sites with full-time operations staffing, the daytime staff will be responsible for off-hours crises too. There is virtually no time that is guaranteed to be totally free of responsibility. Someone needs to be reachable at all times of the day or night in case of emergency, even on holidays and weekends. The best common way to reach the on-call person off-hours is via pager. Pagers are better than cell phones when one member of a team is on call, because the team can make sure that the pager is in the right person s hands, and users need not be concerned with the schedule. ( Let s see, it s Tuesday night. Does Jonathan or Madeline have the pager, or is it Hannah s turn tonight I d check online, but, of course, the system is down. ) Some organizations

Typical af nity values7 for antibody hapten interactions range from 105 to H 1012 M 1 . Because of the very high af nities involved, the reversal of Ab binding may be accomplished only under extreme conditions: at high or low pH values, at high ionic strength, or in the presence of chaotropic ions (e.g., perchlorate) or denaturants (urea, guanidinium) that interfere with hydrogen bonding. Af nity values are experimentally determined by maintaining a constant total Ab concentration, and varying the H concentration. Quantitation of H free and H bound then allows the construction of a Scatchard plot, where H bound = f H free Ab total g is plotted against H bound = Ab total . The slope of this plot is the negative of the af nity, K. Scatchard plots for monoclonal and polyclonal antibodies are shown in Figure 5.4. Note that polyclonal antibodies yield a curved Scatchard plot due to their range of epitope selectivities and af nities.8

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