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report success with Nextel s long-range walkie-talkie system, but for that model to work, a user who might call for support must have a Nextel, or the user must call a central dispatcher who has one. Pagers are simpler (simple is good!), and everyone knows how to get a message to a pager. Other organizations pass around a shared cell phone in the same way they might share a pager; that is a good system, but it can be much more expensive than using a pager, and then the on-call SA will probably be stuck carrying two cell phones. An internal escalation policy must be set into place, with a primary person, a secondary person, and an emergency backup person. If the first person does not respond to a page after a given period of time (often 30 minutes), the backup person is paged and given 30 minutes. After 60 minutes have passed, the third person, usually a manager, is paged. Since the first two people know they will be in trouble if the manager gets paged, it is generally in their best interests to respond quickly.

For polyclonal antibodies, experimental K values represent an average af nity, which is usually represented as K0 55 ANALYTICAL APPLICATIONS OF SECONDARY ANTIBODY ANTIGEN INTERACTIONS 551 Agglutination Reactions Agglutination occurs when an antibody interacts with a multivalent, particulate antigen (ie, an insoluble particle), resulting in cross-linking of the antigen particles by the antibody This eventually leads to clumping, or agglutination, and will not occur with haptens Agglutination may occur when a unideterminate, multivalent antigen (ie, possessing many copies of single epitope A ) interacts with a single antibody (Anti-A); it may also occur if a multideterminate, univalent antigen (with epitopes A, B, C, etc) interacts with at least two distinct antibodies (Anti-A plus Anti-B, eg) Whether or not agglutination occurs depends on the relative concentrations of antigens and antibodies.

On the other hand, in this case, business transformation functions are unimportant, involving only technical transformations to the format (EDI, XML). Selected parameters The following parameters were defined to take account of traffic, service levels, timeslots for opening or SLA and balancing weights.

Very early one August Sunday morning in 1988, the Stern phone rang. My wife, Toby, answered to hear a computer-generated voice, which she promptly hung up on. A few minutes later, the same call, same voice, same hang-up. Who is it I asked. Some computer telling me something about the temperature of a pie, she answered. Voice-generation software being somewhat infantile at the time, I recognized the caller the environmental monitoring system in my startup company s machine room. At 5:30 A.M., the temperature wasn t pie, it was high like over 100 degrees. Our rooftop chiller had failed sometime over the weekend, and the machine room was quickly roasting the disks with the product we planned to introduce at the following week s trade show. One call to the 24-hour HVAC service, 150 feet of garden hose, and a few hundred dollars worth of fans dropped the temperature to the point at which we could safely restore power to the machine room, and by Monday things were back to normal. Moral of the story: When you re on call, your family is on call with you. Make sure that everyone knows how the escalation path reaches you. Hal

In addition to pagers, home phone numbers should be centrally available, and email can also be employed as a backup method Email is the weakest approach in an emergency, especially if the email system is affected by the emergency Even if email is available during the emergency, unlike pagers and cell phones, it requires an action by the recipient to pick it up Late-night phone calls and pages can be reduced through the use of automated processes that take care of the most common and invasive problems But if late-night support work is unavoidable, a tremendous amount of time can be saved by giving your administrators remote access to servers so they can log in from home and correct problems without having to get dressed and drive to the office at all hours of the night Remote access can speed up the initiation of repairs by two hours or more.