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The agglutination test is used to qualitatively test for the presence of antibodies in serum, and is also used in blood grouping As an example, we will consider a test for the presence of antibodies to the bacterium Brucella abortus9 In this test, a series of test tubes are prepared, each containing the same quantity of the bacterial suspension, which is the antigen To each tube is added increasingly dilute solutions of serum If the antibody is present, agglutination will be observed over a range of serum dilutions, as shown in Figure 55 Two dilution zones are apparent, one called the prozone and the other, the agglutination zone In the prozone, no difference is apparent after serum is added and allowed to incubate for several minutes In the agglutination zone, a visible precipitate forms, and settles to the bottom of the tube.

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Many companies pay for their SAs to have high-speed Internet access in their homes via DSL, cable modems, or some alternate method But arrangements must still be made to grant SAs access to the facilities at all hours of the day or night In order to ensure success, SAs must have keys and access cards to the facility and to any important rooms inside, codes to deactivate burglar alarms, emergency phone numbers, appropriate security clearance and passwords, and any access codes to use phone lines at off-hours The bottom line is that your SAs must have complete 24 7 access to any and all parts of the facility that they may need to get to when a crisis is in progress.

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The worst thing that could happen is for your SA to get up at 3:00 in the morning, get dressed, drive to the office, and then be barred access to the systems he needs to get to Never let that happen An occasional dry run, as unpopular as that idea may be, is probably appropriate..

The high-dilution end of the agglutination zone is called the titer of the serum, and is used as a semiquantitative expression of the Ab concentration for the comparison of sera Note that tests made on sera at only one dilution may give misleading results, because of the lack of agglutination in the prozone and at high serum dilutions..

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Ease the burden on your people through the use of automated problem detection and reporting tools. The marketplace is loaded with these sorts of tools; they come from hardware and operating system vendors with their equipment, as well as from vendors who specialize in such products, such as Computer Associates, BMC, and Tivoli. These packages allow users to submit their own problem reports too. Whatever product you use, make sure that trouble tickets and reports include as much detail as possible. These reports need to be reliable, detailed, accurate, verifiable, and documented. The less work an SA has to do to research the problem, the more time she can spend solving it or moving on to the next problem.

Figure 5.5. Agglutination test for Brucella abortus antibodies.9 [Reprinted, with permission, from E. Benjamini, G. Sunshine, and S. Leskowitz, Immunology: A Short Course, 3rd ed., Wiley-Liss, New York, 1996, pp. 115 118. ISBN 0-471-59791-0. Copyright # 1996 by Willey-Liss, Inc.]

Traffic: flow bracket 1 to 1,000 1,000 to 5,000 5,000 to 10,000 > 10,000 Table 10.5. Notation on traffic Note 1 3 5 7

Bad documentation, inadequate testing, and poorly educated users and administrators can be just as damaging to system availability as technical failures. Treat your vendors like people, and in turn they will take better care of you. Realize that your system administration staff is like engine oil. It s only a commodity when it s on the shelf. When they are working for you, it s your responsibility to take care of them. Security, a topic worthy of many books all by itself, is a critical part of system availability.

TABLE 5.2. The Major Human Blood Groupings Blood Group AB A B O Erythrocyte Epitopes Present Both A and B Epitope A Epitope B Neither A nor B Antibodies Present in Serum Neither Anti A nor Anti B Anti-B only Anti-A only Both Anti-A and Anti-B

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