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T. T. Ngo, Ed., Nonisotopic Immunoassay, Plenum Press, New York, 1988. T. T. Ngo and H. M. Lenhoff, Eds., Enzyme-Mediated Immunoassay, Plenum Press, New York, 1985. E. T. Maggio, Enzyme-Immunoassay, CRC Press, Boca Raton, Florida, 1980. R. M. Nakamura, Y. Kasahara, and G. A. Rechnitz, Eds., Immunochemical Assays and Biosensor Technology for the 1990s, American Society for Microbiology, Washington, DC, 1992. C. P. Price and D. J. Newman, Eds., Principles and Practice of Immunoassay, Macmillan (Stockton Press), New York, 1991. D. Wild, Ed., The Immunoassay Handbook, 2nd ed., Nature Publishing Group, New York, 2001.

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Processes accessing files or directories on the failed server are stuck in disk-waits and cannot be killed Even something as innocuous as starting a new shell can hang the client, since the shell walks its directory and command paths They repeat themselves Clients will retransmit requests to the server, after waiting for a timeout period The timeout period doubles after each unanswered request, and after the fifth attempt, you see the NFS server not responding message on the client They eventually get turned upright When the secondary server comes back to life, the client s requests are answered, and it continues where it was previously stuck This assumes you re using hard NFS mounts on your clients 12, Clients and Consumers, discusses the scourge of soft mounts and why you don t want to use them in section Tolerating Data Service Failures.

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Failovers that happen quickly may fly under the radar of the dreaded NFS server not responding messages that signal problems to even the most naive users A server has to stop responding for the better part of 2 minutes for client-side messages to appear If you can complete the failover faster than that, few, if any, users will notice through explicit error messages..

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1. R. M. Nakamura, Y. Kasahara, and G. A. Rechnitz, Eds., Immunochemical Assays and Biosensor Technology for the 1990s, American Society for Microbiology, Washington DC, 1992. 2. P. K. Nakane, Ann. N. Y. Acad. Sci. 254, 1975, 203 211. 3. J. B. Gushaw, M. W. Hu, P. Singh, J. G. Miller, and R. S. Schneider, Clin. Chem. 23, 1977, 1144. 4. B. F. Erlanger, Pharmacol. Rev. 25, 1973, 271 280. 5. B. F. Erlanger, F. Borek, S. M. Beiser, and S. Lieberman, J. Biol. Chem. 228, 1957, 713 727. 6. B. F. Erlanger, F. Borek, S. M. Beiser, and S. Lieberman, J. Biol. Chem. 234, 1959, 1090 1094. 7. D. Exley and R. Abuknesha, FEBS Lett. 79, 1977, 301 304. 8. G. L. Nicolson and S. J. Singer, J. Cell Biol. 60, 1974, 236 248. 9. J. L. Guesdon and S. Avrameas, Immunochemistry 14, 1977, 443 447. 10. R. M. Nakamura, A. Voller, and D. E. Bidwell, in Handbook of Experimental Immunology, Volume 1: Immunochemistry, 4th ed., D. M. Wier, Ed., Oxford (Blackwell Scienti c), New York, 1986. pp. 27.1 27.20. 11. D. Hawcroft, T. Hector, and F. Rowell, Quantitative Bioassay, John Wiley & Sons, Inc., New York, 1984, pp.158 159.

How do you achieve a nirvana in a world dominated by a relatively old network protocol The following are some rules you should follow: NFS should be the only application on the server. NFS is an application, even though it runs in the kernel. Don t mix IP routing or name services or the occasional small database with NFS. First, multiple services make

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failover much more complex, and second, you want your servers concentrated on spitting files out onto the network Performance is a reliability factor, and you should focus on optimizing each server for its intended purpose NIS, NIS+, DHCP, NT domain, and DNS servers belong on other machines Yes, this is a rehash of the previous point, but it s one that is skirted so often that it bears repeating If you re relying on an NIS server running side-by-side with the NFS server, and you need to fail the NFS service to a secondary machine, you re going to have some temporarily unhappy NIS clients waiting to rebind to a server You may accomplish NFS failover quickly but then wait minutes for NIS clients to timeout and rebind to the secondary (or a different) server Separate the servers and spare yourself the headache.

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