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(a) Which of the four species, Ab, Ab*, H and H, must be present at limited H, concentration, and which must be present in excess (b) In addition to the species Ab:H H:Ab*, which other species involving Ab and any of Ab*, H and H will exist on the polystyrene microtiter plates H, following the nal incubation with Ab* and rinsing away of excess reagents

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We ve spent the early part of this chapter lamenting the lack of strict transactional control for client access to shared data in files. When you want transactions, you want a database, and with it comes a new set of challenges for optimizing recovery times, which are explored in the section that follows.

(c) Three potential interferents are shown below in structures D, E, and F (Eq. 6.21). Which of these is likely to present the greatest cross-reactivity in the competitive sandwich immunoassay, and which is expected to present the least cross-reactivity Why

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Databases provide transactional, recoverable access to persistent storage. You can argue about the merits of object-relational or relational or next-generation databases, but the basic mechanisms are the same. Database systems obey the ACID properties: Atomic. Transactions are demarcated and either complete or do not complete. There are no partial insertions or updates. Consistent. All parts of the transaction complete, and all modifications that make up a transaction complete within its scope.

Independent. Transactions do not affect each other, so that one failure is isolated from other successful completions. Durable. Even after a failure, committed data is accessible and correct, while uncommitted or in-flight transactions can be backed out, restarted, or discarded. Atomic and independent transactions are the domain of database management systems. Making sure that the database is available, that it can be recovered quickly, and that it provides a consistent view to clients falls under the availability umbrella, so we focus mainly on the C and D parts of the property sheet consistency and durability.

6:21

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As with NFS servers, it s helpful to know what happens when a database server fails and goes through a takeover cycle, and how clients are likely to react to that chain of events. We look at the usual failover and recovery cases, and then go into the more sinister setting where the failure also causes the database to be corrupted.

23 Consequences for IT Current information systems are therefore composed of applications that were developed in different periods, by different development teams, most of the time without real coordination, and using different technologies In the majority of cases, new applications were created using the most recent technology at the time, often without genuinely questioning the necessity of implementing such a technology, and forgetting that a modern technology becomes obsolete in an instant of time In addition, as we have seen, these applications were not originally designed to communicate with each other The links and interfaces that allow them to cooperate had to be created between each application The introduction of commercial software packages in information systems has only reinforced this problem, since they were designed to respond to a particular problem without being preoccupied about communicating with the existing system.

The first order of business is to be sure you truly have a database failure and not merely a break in the action. Some database transactions can be intense and long-running, making the server appear to be unresponsive when it s crunching through millions of records. Unlike NFS clients, who resend requests that appear to have vaporized, database clients have to respect state, transaction boundaries, and sequencing that are enforced by the ACID properties. It would occasionally be nice to have the ever-popular interest rate calculation example (where an application applies interest to some accounts, crashes, and then starts over again recrediting interest to the first few accounts updated before the crash) performed a few dozen times on your bank account, but database application designers are smarter than that. In the cases where the IT people fail, the bean counters pick up the error; there s always another level of management. You want your failover management system to check several test points in the database. Listener processes or back-end reader/writer processes may fail, leaving the data incommunicado with the server; or the database engine may seize up or the SQL interpreter fail, allowing the database to receive requests but not do anything useful with them. It s a good idea to check the operation of the database, using a short piece of SQL that looks up values in a small table reserved

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