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Frequent state updates. Replication may fall behind the current user session, since each transfer of state between application servers is a transactional update to memory, which is faster than disk, but still involves significant handshaking overhead. Advantage: database. Recovery time. If your database fails, your application servers pause while it recovers; your MTTR for the web site is at least as long as the MTTR for the database server. Without a database, the MTTR is much shorter because you only have to wait for the web server layer to start redirecting requests to alternative application servers. Advantage: application server. Large numbers of sessions. Each one is doing inserts and updates in your database. Tuning of the database is critical, unless you prune down the state to make replication sufficient. Advantage: slight edge to application server. Performance. Avoiding the database query is going to be a win; using memory as a cache for the interesting data will keep you running at memory speeds. Advantage: application server. Long-term persistence. Want your users to find that saved item from three months ago You don t want the equivalent of plaque building up in your application servers. Write it to a database and retrieve it based on a user cookie. Advantage: database. Scale. In a pure database-centric state management solution, you scale by adding another application server that s a client of the database. On the other hand, you need to scale your database to handle the additional number of users, sessions, and queries. Advantage: the solution with the most manageable complexity, as complexity is going to introduce points of administrative failure. There are no cut-and-dried rules for making the web-based services routing sequence reliable; you need to balance your requirements for scale, recovery time, and real-time performance.

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A directory server is a database-like, networked repository that is optimized for hierarchical, read-mostly access to its data. Directory servers are frequently referred to as Lightweight Directory Access Protocol (LDAP) servers, although technically LDAP is the protocol that is used to talk to the server that contains the directory. Directory servers are used most commonly in the web-based services realm as places to store configuration data for the web site, user registration information, and other data that needs to be shared by many access points.

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channel created through the membrane Concentration gradients of sodium and potassium ions exist across membranes in vivo, and an open ion channel allows the ow of ions, and therefore an ion current exists across the membrane The AcChR is isolated from the electroplax tissue of electric eels and rays, where it is found at high levels The isolated receptors bind AcCh and its analogues, as well as a-bungarotoxin (BTX, a protein isolated from snake venom) and neurotoxins such as tubocurarine The receptor behaves like an antibody in that it has selective-binding sites, but it possesses no catalytic properties It may be immobilized by standard protein immobilization methods The AcCh biosensor uses competitive binding between FITC labeled or unlabeled bungarotoxin and receptor immobilized by adsorption onto the surface of a quartz optical ber.

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Why not just use a database for everything Directories are optimized for reading, so they will typically provide better performance in the real-time world of the Web. Directory servers also use replication between a master server and several slaves, making reliability a function of choosing any available server. Directories also have a hierarchical (tree) structure, allowing entries to be organized to match organization charts, product data, or other data that fits the tabular or table-of-tables model. Databases excel at transactional integrity, high-volume updates, and inserts, so typically you ll find both directories and repositories behind the web and application server fa ade of a web site. The following are some considerations for making directory servers reliable:

Determine what the rate is of updates and the volume of updates. Directory servers replay updates from the master to the slaves, and high volumes of updates may slow down normal directory service. Ensure that you have enough slave servers to continue to provide high performance lookups even if one or more slave servers have crashed. Validate that the window of inconsistency the longest time period from when you make a directory update until that update is reflected in all slave servers is within the bounds of what your web-based applications can tolerate. If applications want strong read-back semantics that is, they want to see changes reflected in the data repository as soon as they re made you re probably going to need to use a database for the rapidly changing, instant-replay data.

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