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Three zones are apparent in the curve shown in Figure 5.6. At low antigen concentrations, there is a zone of antibody excess, where antibody behaves univalently. In the equivalence zone, antigen and antibody are present in roughly equal proportions, and the largest quantity of precipitin forms. At higher antigen concentrations, the antigen behaves univalently, and its excess does not allow the formation of a highly cross-linked, insoluble network of precipitin. The precipitin reaction can be induced in a gel, allowing qualitative tests for the presence of certain antibodies in serum. The gels used are often agar, and are cast on a at glass plate, such as a microscope slide. The double-diffusion method12 uses such a cast gel, with two wells cut into the gel, one at each end of the slide. The unknown serum is placed in one well, and the test antigen solution is placed in the other. Both solutions diffuse into the gel, and set up two opposing concentration gradients between the wells. If the suspected antibody is present in the serum, then at some distance between the wells, the Ab/Ag concentration ratio will be optimal for precipitin formation. As shown in Figure 5.7, a precipitin line forms at Ab/ Ag equivalence. Provided that the diffusion rates of different antigens in the gel are known, a multiple test may be conducted on a single slide, with the positions of the precipitin lines indicating which antibodies are present in the serum sample.

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The barcode scanner project is interesting because the Intel Edison has wi-fi built in. ... The open source Kaazing Gateway includes Java client libraries.

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12 Jun 2007 ... Business Refinery Barcode Reader for Java , a library to create barcode, supports Linear (1D), PDF417 (2D), Data Matrix. Barcode Reader for ...

Another way to look at the math is to consider that for all practical purposes, the seven components will never be down at the same time. Since each component will be responsible for 52 minutes of downtime per year (based on 99.99 percent availability), 7 times 52 is 364 minutes, or just over 6 hours per year, or 99.93 percent. The actual path from user to servers is going to be much more complicated than the one in Figure 2.1. For example, the network cloud is made up of routers, hubs, and switches, any of which could fail and thereby lower network availability. If the storage is mirrored, then its availability will likely be higher, but the value will surely vary. The formulas also exclude many other components that could cause additional downtime if they were to fail, such as electric power or the building itself. Consider another example. Six of the seven components in the chain deliver 99.99 percent availability, but the seventh only achieves 99 percent uptime. The overall availability percentage for that chain of components will be just 98.94 percent. Great returns on investment can be achieved by improving the availability of that weakest link. So, while some single components may be able to deliver upwards of 99.99 percent availability, it is much more difficult for an entire system, from user to server, to deliver the same level. The more components there are in the chain and the more complex the chain, the lower the overall availability will be. Any bad component in the chain can lower overall availability, but there is no way for one good component to raise it above the level of the weakest link.

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SLA 5-day Coverage (9:00 to 18:00) 7-day Coverage (9:00 to 18:00) Non-stop Coverage Table 10.7. Notation on SLA

Figure 5.7. The double-diffusion method for (a) a single Ab and (b) multiple antibodies. In (b) the antigens diffuse at different rates with 1 > 2 > 3.

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Figure 5.8. Double-diffusion test of antigen identity: (a) identity, (b) nonidentity, and (c) partial identity.

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